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ミヤイ サヤカ
Sayaka Miyai
宮井 紗弥香 所属 動物看護学部 動物看護学科 職種 講師 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2012/03 |
| 形態種別 | 学術雑誌 |
| 査読 | 査読あり |
| 標題 | Biochemical characterization of novel lignans isolated from the wood of Taxus yunnanensis as effective stimulators for GSK-3b and the phosphorylation of basic brain proteins by the kinase in vitro. |
| 執筆形態 | 共著 |
| 掲載誌名 | Biol. Pharm. Bull., 35(3), 320-329 (2012) |
| 著者・共著者 | Kenzo Ohtsuki, Sayaka Miyai, Akira Yamaguchi, Kouhei Morikawa, and Tetsuroh Okano |
| 概要 | The stimulatory and inhibitory effects of several compounds and lignans isolated from the water extract of Taxus yunnanensis on the phosphorylation of three functional brain proteins (bMBP, rhTP and rCRMP-2) by glycogen synthase kinase-3β (GSK-3β) were quantitatively compared in vitro, using (-)EGCG as a positive control. We found that (i) three selected Taxus lignans highly stimulated the autophosphorylation of GSK-3β and the GSK-3β-mediated phosphorylation of two basic brain proteins [bMBP and rhTP], but inhibited dose-dependently the phosphorylation of an acidic protein (rCRMP-2) by the kinase; (ii) these three Taxus lignans showed binding-affinities with bMBP as well as rhTP, but had low affinities with rCRMP-2; (iii) the binding of tanegool and (7R)-7-hydroxytaxiresinol to these two basic proteins induced their novel potent phosphorylation sites for GSK-3β; and (iv) these three Taxus lignans, but not EGCG, induced Tyr-phosphorylation of GSK-3β in vitro. |