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ナカムラ ケンスケ
KENSUKE NAKAMURA
中村 建介 所属 前橋工科大学 工学部 生命情報学科 前橋工科大学大学院 工学研究科 生命情報学専攻（修士課程） 前橋工科大学大学院 工学研究科 環境・生命工学専攻（博士課程） 前橋工科大学 工学部 情報・生命工学群 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2018 |
| 形態種別 | 記事・総説・解説・論説等（学術雑誌） |
| 査読 | 査読あり |
| 標題 | GeF-seq: A Simple Procedure for Base Pair Resolution ChIP-seq |
| 執筆形態 | 共著 |
| 掲載誌名 | Bacterial Chromatin |
| 掲載区分 | 国外 |
| 出版社・発行元 | Humana Press, New York, NY |
| 巻・号・頁 | pp.33-47 |
| 概要 | Nucleotide sequences recognized and bound by DNA-binding proteins (DBPs) are critical to control and maintain gene expression, replication, chromosome segregation, cell division, and nucleoid structure in bacterial cells. Therefore, determination of the binding sequences of DBPs is important not only to study DBP recognition mechanisms, but also to understand the fundamentals of cell homeostasis. While ChIP-seq analysis appears to be an effective way to determine DBP-binding sites on the genome, the resolution is sometimes not sufficient to identify the sites precisely. Here, we introduce a simple and effective method named Genome footprinting with high-throughput sequencing (GeF-seq) to determine binding sites of DBPs at single base-pair resolution. GeF-seq detects binding sites of DBPs as sharp peaks and thus makes it possible to identify the recognition sequence in each “binding peak |