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Osamu HIRUTA
Department Kumamoto Health Science University , Kumamoto Health Science University Graduate School Division of Health Sciences, Graduate School of Health Sciences Position |
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| Language | English |
| Publication Date | 1991/06 |
| Type | |
| Peer Review | Peer reviewed |
| Title | Accumulation of Bialaphos Intermediates under High Oxygen Partial Pressure and Their Conversion to Bialaphos : Studies on the Production of Bialaphos(IV). |
| Contribution Type | |
| Journal | Journal of fermentation and bioengineering |
| Journal Type | Japan |
| Publisher | Society for Biotechnology, Japan |
| Volume, Issue, Page | 71(6),pp.433-435 |
| Author and coauthor | Hidehi Takebe, Osamu Hiruta, Mihoko Matsunaga, Atsuyuki Satoh, Hideo Tanaka. |
| Details | In bialaphos fermentation by Streptomyces hygroscopicus, production of bialaphos was reduced when the partial pressure of oxygen was increased. The culture filtrate was analyzed revealing that two possible biosynthetic intermediates of bialaphos were accumulated. These two compounds were identified to be C-P compounds having an amino group, one as a demethyl derivative of bialaphos (MP-102) and the other a demethyl derivative of AMPB [dealaninated bialaphos] (MP-101). It was also demonstrated that these demethyl derivatives were converted to bialaphos under low oxygen partial pressure, whereas the conversion was suppressed under high oxygen partial pressure. As a conclusion, methylation at the final step of the biosynthetic process of bialaphos did not proceed under high oxygen partial pressure, resulting in a low bialaphos concentration due to the accumulation of the demethyl derivatives. |