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Osamu HIRUTA
Department Kumamoto Health Science University , Kumamoto Health Science University Graduate School Division of Health Sciences, Graduate School of Health Sciences Position |
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| Language | English |
| Publication Date | 1996/02 |
| Type | |
| Peer Review | Peer reviewed |
| Title | γ-Linolenic Acid Production by a Low Temperature-Resistant Mutant of Mortierella ramanniana |
| Contribution Type | |
| Journal | Journal of fermentation and bioengineering |
| Journal Type | Japan |
| Volume, Issue, Page | 82(2),pp.119-123 |
| Total page number | 5 |
| Author and coauthor | Osamu Hiruta, Yasushi Kamisaka, Toshihiro Yokochi, Takafumi Futamura, Hidehi Takebe, Atsuyuki Satoh, Toro Nakahara, Osamu Suzuki |
| Details | As a result of breeding experiments using low temperature growth as an index, Mortierella ramanniana mutant MM 15-1, which produces lipids with a high γ-linolenic acid (GLA) content, was obtained. The results of cultivation experiments in Erlenmeyer flasks showed that although the total lipid produced by the MM15-1 mutant was about 14% lower than that of the parent strain (M. ramanniana IFO 8187), the GLA content of the lipid was 16.5%, which represents about a 2-fold increase over that of the parent strain. In particular, there was a significant increase in the GLA proportion of the phospholipid fraction in the MM15-1 mutant, especially in the phosphatidylethanolamine (PE) and phosphatidylinositol (PI). When the MM 15-1 mutant was cultivated in a 600-l fermentor, it produced lipid with 18.3% GLA. This was a 2-fold higher than the value obtained with the parent strain under the same cultivation conditions. |