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ヒルタ オサム
Osamu HIRUTA
蛭田 修 所属 熊本保健科学大学 品質保証・精度管理学共同研究講座 熊本保健科学大学大学院 保健科学研究科 保健科学専攻 職位 特命教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 1996/02 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | γ-Linolenic Acid Production by a Low Temperature-Resistant Mutant of Mortierella ramanniana |
| 執筆形態 | 共著 |
| 掲載誌名 | Journal of fermentation and bioengineering |
| 掲載区分 | 国内 |
| 巻・号・頁 | 82(2),pp.119-123 |
| 総ページ数 | 5 |
| 著者・共著者 | Osamu Hiruta, Yasushi Kamisaka, Toshihiro Yokochi, Takafumi Futamura, Hidehi Takebe, Atsuyuki Satoh, Toro Nakahara, Osamu Suzuki |
| 概要 | As a result of breeding experiments using low temperature growth as an index, Mortierella ramanniana mutant MM 15-1, which produces lipids with a high γ-linolenic acid (GLA) content, was obtained. The results of cultivation experiments in Erlenmeyer flasks showed that although the total lipid produced by the MM15-1 mutant was about 14% lower than that of the parent strain (M. ramanniana IFO 8187), the GLA content of the lipid was 16.5%, which represents about a 2-fold increase over that of the parent strain. In particular, there was a significant increase in the GLA proportion of the phospholipid fraction in the MM15-1 mutant, especially in the phosphatidylethanolamine (PE) and phosphatidylinositol (PI). When the MM 15-1 mutant was cultivated in a 600-l fermentor, it produced lipid with 18.3% GLA. This was a 2-fold higher than the value obtained with the parent strain under the same cultivation conditions. |