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Osamu HIRUTA
Department Kumamoto Health Science University , Kumamoto Health Science University Graduate School Division of Health Sciences, Graduate School of Health Sciences Position |
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| Language | English |
| Publication Date | 1996/04 |
| Type | |
| Peer Review | Peer reviewed |
| Title | Optimization and Scale-Up of γ-Linolenic Acid Production by Mortierella ramanniana MM 15-1,a High γ-Linolenic Acid Producing Mutant |
| Contribution Type | |
| Journal | Journal of fermentation and bioengineering |
| Journal Type | Japan |
| Volume, Issue, Page | 82(4),pp.366-370 |
| Author and coauthor | Osamu Hiruta, Takafumi Futamura, Hidehi Takebe, Atsuyuki Satoh, Yasushi Kamisaka, Toshihiro Yokochi, Toro Nakahara, Osamu Suzuki |
| Details | Optimum conditions for cultivation of Mortierella ramanniana mutant MM 15-1 were investigated with an aim of producing a lipid which contains high γ-linolenic acid (GLA). Although both pellet and filamentous forms of this mutant were observed during the cultivation, the pellets accumulated lipid with higher GLA content than the filamentous forms. The effects of culture conditions on pellet formation were therefore investigated in a 30-l jar fermentor. Furthermore, when this process was scaled up to a 10-kl fermentor, using the impeller tip velocity as the scale-up parameter, both the pellet formation and the lipid production as well as the GLA content of the lipid were consistent with those obtained in the 30-l jar fermentor. |