|
ヒルタ オサム
Osamu HIRUTA
蛭田 修 所属 熊本保健科学大学 品質保証・精度管理学共同研究講座 熊本保健科学大学大学院 保健科学研究科 保健科学専攻 職位 特命教授 |
|
| 言語種別 | 英語 |
| 発行・発表の年月 | 1998 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Enzymatic degradation of poly(hydroxyalkanoate) by Corynebacterium aquaticum IM-1 isolated from activated sludge |
| 執筆形態 | 共著 |
| 掲載誌名 | Polymer Degradation and Stability |
| 掲載区分 | 国外 |
| 巻・号・頁 | 61(2) |
| 著者・共著者 | Masako Ito, Yuji Saito, Toshio Matsunobu, Osamu Hiruta, Hidehi Takebe |
| 概要 | Poly(4-hydroxybutyric acid)-degrading bacteria were isolated from activated sludge. One strain was selected, which was identified as Corynebacterium aquaticum. The strain C. aquaticum IM-1 excreted an extracellular poly(hydroxyalkanoate) [PHA]depolymerase and grew on 4-hydroxybutyric acid as the sole carbon source. The molecular weight of the purified PHA depolymerase was determined as approximately 33000 Da by SDS-PAGE. The optimum activity of the depolymerase was observed at pH 6.5 and 40 °C. and N-terminal sequences of the depolymerase were not applicable to the well-known enzymes. The enzymatic degradation of polyesters was studied by the erosion rate of solution-cast films of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) and poly(ω-hydroxyalkanoate) of different chain lengths (C3–C6). The water-soluble products of poly(3-hydroxybutyrateco-97mol%4-hydroxybutyrate) film were revealed by 1H NMR and FAB-MS analysis, and the main product was the dimer of 4-hydroxybutyric acid. |