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Osamu HIRUTA
Department Kumamoto Health Science University , Kumamoto Health Science University Graduate School Division of Health Sciences, Graduate School of Health Sciences Position |
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| Language | English |
| Publication Date | 2002 |
| Type | |
| Peer Review | Peer reviewed |
| Title | Factors affecting the composition of oligosaccharides produced in chitosan hydrolysis using immobilized chitosanases |
| Contribution Type | |
| Journal | Biotechnology Progress |
| Journal Type | Another Country |
| Volume, Issue, Page | 18(5),pp.969-974 |
| Total page number | 6 |
| Author and coauthor | Takashi Kuroiwa, Sosaku Ichikawa, Osamu Hiruta, Seigo Sato, Sukekuni Mukataka |
| Details | The hydrolysis reaction of chitosan using immobilized chitosanases with regard to the composition of its products and the yield of the intermediate target products, pentamer and hexamer of chitosan oligosaccharides, was investigated. Chitosanase was immobilized onto agar or agarose gel particles by the multipoint attachment method. In batch experiments, surface enzyme density, support particle size, temperature, agitator speed, and initial substrate concentration significantly affected the composition of the oligosaccharides produced. It was believed that these factors all related to the reaction rate and mass transfer rate at the surface of the support materials immobilizing the enzymes. These effects were summarized as a correlation with Damköhler number (Da), defined as the ratio of the maximum reaction rate to the maximum mass transfer rate. The result showed that the reaction conditions that give a low value of Da provide a high yield of pentamer and hexamer oligosaccharides. |