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SAKAMOTO Chiyomi
Department Kumamoto Health Science University , Position |
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| Language | English |
| Publication Date | 2016 |
| Type | |
| Title | Loss of the integral nuclear envelope protein SUN1 induces alteration of nucleoli |
| Contribution Type | |
| Journal | Nucleus |
| Journal Type | Another Country |
| Responsible for | Quantitation of morphological differences |
| Author and coauthor | Matsumoto A, Sakamoto C, Matsumori H, Katahira J, Yasuda Y, Yoshidome 4, Tsujimoto M, Goldberg IG, Matsuura N, Nakao M, Saitoh N, Hieda M |
| Details | We used the algorithm wndchrm to quantify the effects on nucleolar morphology of the loss of the components of nuclear envelope in a human mammary epithelial cell line.We found that a reduction in the levels of SUN1, SUN2, and lamin A/C led to significant changes in morphologies that were computationally classified using wndchrm with approximately 100% accuracy. In particular, depletion of SUN1 caused nucleolar hypertrophy and reduced rRNA synthesis. Further, wndchrm revealed a consistent negative correlation between SUN1 expression and the size of nucleoli in human breast cancer tissues. Our unbiased morphological quantitation strategies using wndchrm revealed an unexpected link between the components of the LINC complex and the morphologies of nucleoli that serves as an indicator of the malignant phenotype of breast cancer cells. |