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サカモト チヨミ
SAKAMOTO Chiyomi
坂本 智代美 所属 熊本保健科学大学 生物毒素・抗毒素共同研究講座 職位 特命助教 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2016 |
| 形態種別 | 研究論文 |
| 標題 | Loss of the integral nuclear envelope protein SUN1 induces alteration of nucleoli |
| 執筆形態 | 共著 |
| 掲載誌名 | Nucleus |
| 掲載区分 | 国外 |
| 担当範囲 | Quantitation of morphological differences |
| 著者・共著者 | Matsumoto A, Sakamoto C, Matsumori H, Katahira J, Yasuda Y, Yoshidome 4, Tsujimoto M, Goldberg IG, Matsuura N, Nakao M, Saitoh N, Hieda M |
| 概要 | We used the algorithm wndchrm to quantify the effects on nucleolar morphology of the loss of the components of nuclear envelope in a human mammary epithelial cell line.We found that a reduction in the levels of SUN1, SUN2, and lamin A/C led to significant changes in morphologies that were computationally classified using wndchrm with approximately 100% accuracy. In particular, depletion of SUN1 caused nucleolar hypertrophy and reduced rRNA synthesis. Further, wndchrm revealed a consistent negative correlation between SUN1 expression and the size of nucleoli in human breast cancer tissues. Our unbiased morphological quantitation strategies using wndchrm revealed an unexpected link between the components of the LINC complex and the morphologies of nucleoli that serves as an indicator of the malignant phenotype of breast cancer cells. |