サカモト チヨミ   SAKAMOTO Chiyomi
  坂本 智代美
   所属   熊本保健科学大学  生物毒素・抗毒素共同研究講座
   職位   特命助教
言語種別 英語
発行・発表の年月 2017/10/15
形態種別 研究論文 
標題 Condensin II plays an essential role in reversible assembly of mitotic chromosomes in situ
執筆形態 共著
掲載誌名 Molecular Biology of the Cell
掲載区分国外
担当範囲 Morphological quantification of chromatin and axes
著者・共著者 Ono T, Sakamoto C, Nakao M, Saitoh N, Hirano T
概要 we have established a set of two-step protocols for inducing reversible assembly of chromosome structure in situ, namely within a whole cell. This assay combined with small interfering RNA depletion demonstrates that the recovery of chromatin shapes and the reorganization of axes are highly sensitive to depletion of condensin II but less sensitive to depletion of condensin I or topoisomerase IIα. Furthermore, quantitative morphological analyses using the machine-learning algorithm wndchrm support the notion that chromosome shaping is tightly coupled to the reorganization of condensin II-based axes. We propose that condensin II makes a primary contribution to mitotic chromosome architecture and maintenance in human cells.