|
Rui Yamaguchi
Department Kumamoto Health Science University Department of Medical Technology, Faculty of Health Science Kumamoto Health Science University Graduate School Division of Health Sciences, Graduate School of Health Sciences Position Associate Professor |
|
| Language | English |
| Publication Date | 2011/03 |
| Type | |
| Peer Review | Peer reviewed |
| Title | Salt-dependent thermo-reversible alpha-amylase: cloning and characterization of halophilic alpha-amylase from moderately halophilic bacterium, Kocuria varians. |
| Contribution Type | |
| Journal | Appl Microbiol Biotechnol. |
| Journal Type | Another Country |
| Volume, Issue, Page | 89,pp.673-684 |
| Total page number | 11 |
| Authorship | Lead author |
| Author and coauthor | Yamaguchi R, Tokunaga H, Ishibashi M, Arakawa T, Tokunaga M |
| Details | A moderately halophilic bacterium, Kocuria varians, was found to produce active α-amylase (K. varians α-amylase (KVA)). We have observed at least six different forms of α-amylase secreted by this bacterium into the culture medium. Characterization of these KVA forms and cloning of the corresponding gene revealed that KVA comprises pre-pro-precursor form of α-amylase catalytic domain followed by the tandem repeats, which show high similarity to each other and to the starch binding domain (SBD) of other α-amylases. |