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Rui Yamaguchi
Department Kumamoto Health Science University Department of Medical Technology, Faculty of Health Science Kumamoto Health Science University Graduate School Division of Health Sciences, Graduate School of Health Sciences Position Associate Professor |
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| Language | English |
| Publication Date | 2012/01 |
| Type | |
| Peer Review | Peer reviewed |
| Title | Halophilic properties of metal binding protein characterized by high histidine content from Chromohalobacter salexigens DSM3043. |
| Contribution Type | |
| Journal | Protein J. |
| Journal Type | Another Country |
| Volume, Issue, Page | 31,pp.175-183 |
| Total page number | 8 |
| Authorship | Lead author |
| Author and coauthor | Yamaguchi R, Arakawa T, Tokunaga H, Ishibashi M, Tokunaga M. |
| Details | Periplasmic metal binding protein characterized by high histidine content was cloned from moderate halophile, Chromohalobacter salexigens. The protein, termed histidine-rich metal binding protein (HP), was expressed in and purified from E. coli as a native form. HP bound to Ni- and Cu-loaded chelate columns with high affinity, and Co- and Zn-columns with moderate affinity. Although the secondary structure was not grossly altered by the addition of 0.2–2.0 M NaCl, the thermal transition pattern was considerably shifted to higher temperature with increasing salt concentration. |