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ヤマグチ ルイ
Rui Yamaguchi
山口 類 所属 熊本保健科学大学 保健科学部 医学検査学科 熊本保健科学大学大学院 保健科学研究科 保健科学専攻 職位 准教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2012/01 |
| 形態種別 | 研究論文（学術雑誌） |
| 査読 | 査読あり |
| 標題 | Halophilic properties of metal binding protein characterized by high histidine content from Chromohalobacter salexigens DSM3043. |
| 執筆形態 | 共著 |
| 掲載誌名 | Protein J. |
| 掲載区分 | 国外 |
| 巻・号・頁 | 31,pp.175-183 |
| 総ページ数 | 8 |
| 担当区分 | 筆頭著者 |
| 著者・共著者 | Yamaguchi R, Arakawa T, Tokunaga H, Ishibashi M, Tokunaga M. |
| 概要 | Periplasmic metal binding protein characterized by high histidine content was cloned from moderate halophile, Chromohalobacter salexigens. The protein, termed histidine-rich metal binding protein (HP), was expressed in and purified from E. coli as a native form. HP bound to Ni- and Cu-loaded chelate columns with high affinity, and Co- and Zn-columns with moderate affinity. Although the secondary structure was not grossly altered by the addition of 0.2–2.0 M NaCl, the thermal transition pattern was considerably shifted to higher temperature with increasing salt concentration. |