Rui Yamaguchi
Department Kumamoto Health Science University Department of Medical Technology, Faculty of Health Science Kumamoto Health Science University Graduate School Division of Health Sciences, Graduate School of Health Sciences Position Associate Professor |
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Language | English |
Publication Date | 2015/12 |
Type | |
Peer Review | Peer reviewed |
Title | Halophilic starch-binding domain as a novel fusion protein partner for efficient recombinant protein expression |
Contribution Type | |
Journal | Bull Soc Sea Water Sci. |
Journal Type | Japan |
Volume, Issue, Page | 69,pp.384-385 |
Total page number | 1 |
Author and coauthor | Yamaguchi R, Ishibashi M, Tokunaga H, Arakawa T, Tokunaga M |
Details | Halophiles produce halophilic enzymes which function well in high salinity conditions. The starch binding domain(SBD)of halophilic α-amylase has been found to show typical halophilic characteristics with high aqueous solubility and folding capacity. Here we developed a fusion protein expression vector, pSBF-S1, using this SBD as a fusion partner, looking for highly soluble protein expression and efficient affinity-purification. The fusion protein expressed could be purified by dual affinity chromatographies, i.e. Ni-affinity resin with N-terminal His-tag and amylose-resin with SBD affinity. The target protein is then generated through thrombin-digestion of fusion protein at the cleavage site between the target protein and SBD. |