ヤマグチ ルイ   Rui Yamaguchi
  山口 類
   所属   熊本保健科学大学  保健科学部 医学検査学科
   熊本保健科学大学大学院  保健科学研究科 保健科学専攻
   職位   准教授
言語種別 英語
発行・発表の年月 2015/12
形態種別 記事・総説・解説・論説等(学術雑誌)
査読 査読あり
標題 Halophilic starch-binding domain as a novel fusion protein partner for efficient recombinant protein expression
執筆形態 共著
掲載誌名 Bull Soc Sea Water Sci.
掲載区分国内
巻・号・頁 69,pp.384-385
総ページ数 1
著者・共著者 Yamaguchi R, Ishibashi M, Tokunaga H, Arakawa T, Tokunaga M
概要 Halophiles produce halophilic enzymes which function well in high salinity conditions. The starch binding domain(SBD)of halophilic α-amylase has been found to show typical halophilic characteristics with high aqueous solubility and folding capacity. Here we developed a fusion protein expression vector, pSBF-S1, using this SBD as a fusion partner, looking for highly soluble protein expression and efficient affinity-purification. The fusion protein expressed could be purified by dual affinity chromatographies, i.e. Ni-affinity resin with N-terminal His-tag and amylose-resin with SBD affinity. The target protein is then generated through thrombin-digestion of fusion protein at the cleavage site between the target protein and SBD.