Rui Yamaguchi
Department Kumamoto Health Science University Department of Medical Technology, Faculty of Health Science Kumamoto Health Science University Graduate School Division of Health Sciences, Graduate School of Health Sciences Position Associate Professor |
|
Language | English |
Publication Date | 2016/02 |
Type | |
Peer Review | Peer reviewed |
Title | Halophilic metal binding protein and its His-Asp repeating peptides as fusion partner for high solubility-purification of fusion proteins |
Contribution Type | |
Journal | Bull Soc Sea Water Sci. |
Journal Type | Japan |
Volume, Issue, Page | 70,pp.51-52 |
Total page number | 1 |
Authorship | Lead author |
Author and coauthor | Yamaguchi R, Ishibashi M, Tokunaga H, Arakawa T, Tokunaga M |
Details | Histidine-rich metal binding protein(HP)has been found to have high binding capacity to several heavy metal ions and was expressed in Escherichia coli cytoplasm as soluble form in large amount. Here, we employed properties of this halophilic HP as a solubility-enhancing partner protein for soluble expression and for immobilized metal ion affinity purification. We also demonstrated that a novel insertion sequence(HD50)of HP containing His-Asp repeating sequence exhibited metal binding capacity. |