|
Rui Yamaguchi
Department Kumamoto Health Science University Department of Medical Technology, Faculty of Health Science Kumamoto Health Science University Graduate School Division of Health Sciences, Graduate School of Health Sciences Position Associate Professor |
|
| Language | English |
| Publication Date | 2016/01 |
| Type | |
| Peer Review | Peer reviewed |
| Title | Human neutrophil elastase induce IL-10 expression in PBMC through PKC
theta/delta and phospholipase pathways |
| Contribution Type | |
| Journal | Cell J. |
| Journal Type | Another Country |
| Volume, Issue, Page | 17,pp.692-700 |
| Total page number | 8 |
| Author and coauthor | Kawata J, Yamaguchi R, Yamamoto T, Ishimaru Y, Sakamoto A, Aoki M, Kitano M, Umehashi M, Hirose E, Yamaguchi Y |
| Details | A phospholipase C inhibitor (U73122) partially blunt- ed the induction of IL-10 mRNA expression by HNE, while IL-10 mRNA expression was significantly reduced by a protein kinase C (PKC) inhibitor (Rottlerin). A calcium chelator (3,4,5-trimethoxybenzoic acid 8-(diethylamino)octyl ester: TMB-8) inhibited the response of IL-10 mRNA to stimulation by HNE. In addition, pretreatment with a broad-spectrum PKC inhibitor (Ro-318425) partly blocked the response to HNE. Finally, an inhibitor of PKC theta/delta abolished the increased level of IL-10 mRNA expression.These results indicate that HNE mainly upregulates IL-10 mRNA ex- pression and protein production in moncytes via a novel PKC theta/delta, although partially via the conventional PKC pathway. |