ヤマグチ ルイ   Rui Yamaguchi
  山口 類
   所属   熊本保健科学大学  保健科学部 医学検査学科
   熊本保健科学大学大学院  保健科学研究科 保健科学専攻
   職位   准教授
言語種別 英語
発行・発表の年月 2016/01
形態種別 研究論文(学術雑誌)
査読 査読あり
標題 Human neutrophil elastase induce IL-10 expression in PBMC through PKC
theta/delta and phospholipase pathways
執筆形態 共著
掲載誌名 Cell J.
掲載区分国外
巻・号・頁 17,pp.692-700
総ページ数 8
著者・共著者 Kawata J, Yamaguchi R, Yamamoto T, Ishimaru Y, Sakamoto A, Aoki M, Kitano M, Umehashi M, Hirose E, Yamaguchi Y
概要 A phospholipase C inhibitor (U73122) partially blunt- ed the induction of IL-10 mRNA expression by HNE, while IL-10 mRNA expression was significantly reduced by a protein kinase C (PKC) inhibitor (Rottlerin). A calcium chelator (3,4,5-trimethoxybenzoic acid 8-(diethylamino)octyl ester: TMB-8) inhibited the response of IL-10 mRNA to stimulation by HNE. In addition, pretreatment with a broad-spectrum PKC inhibitor (Ro-318425) partly blocked the response to HNE. Finally, an inhibitor of PKC theta/delta abolished the increased level of IL-10 mRNA expression.These results indicate that HNE mainly upregulates IL-10 mRNA ex- pression and protein production in moncytes via a novel PKC theta/delta, although partially via the conventional PKC pathway.