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Misa Haraguchi
Department Kumamoto Health Science University Department of Medical Technology, Faculty of Health Science Position Assistant Professor |
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| Language | English |
| Publication Date | 2018/08 |
| Type | |
| Peer Review | Peer reviewed |
| Title | Transcription factor specificity protein 1 modulates TGFβ1/Smad signaling to negatively regulate SIGIRR expression by human M1 macrophages stimulated with substance P |
| Contribution Type | |
| Journal | Cytokine |
| Journal Type | Another Country |
| Volume, Issue, Page | 108,pp.24-36 |
| Total page number | 13 |
| Responsible for | 本人担当部分：共同研究により抽出不可 |
| Author and coauthor | Yamaguchi R, Sakamoto A, Yamaguchi R, Haraguchi M, Narahara S, Sugiuchi H,Yamaguchi Y |
| Details | The stimuli inducing expression of single immunoglobulin IL-1-related receptor (SIGIRR) and the relevant regulatory mechanisms are not well defined. Transforming growth factor β1 (TGFβ1) delays internalization of neurokinin-1 receptor (NK1R) and subsequently enhances cellular signaling. This study investigated the effect of TGFβ1 on SIGIRR protein production by human M1 macrophages in response to stimulation with substance P (SP). SP caused upregulation of SIGIRR expression in a concentration-dependent manner, whereas aprepitant (an NK1R inhibitor) blunted this response. Silencing p38γMAPK or TAK-1 partially attenuated the response to SP stimulation, while TGFβ1/2/3 siRNA dramatically diminished it. SP induced much greater SIGIRR protein production than either lipopolysaccharide (a TLR4 agonist) or resiquimod (a TLR7/8 agonist). |