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Ippei noboruo
Department Kumamoto Health Science University Department of Medical Technology, Faculty of Health Science Position Assistant Professor |
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| Language | Japanese |
| Publication Date | 2022/03 |
| Type | |
| Peer Review | Peer reviewed |
| Title | A basic study to establish a new method to avoid the effects of daratumumab in indirect antiglobulin test |
| Contribution Type | |
| Journal | Journal of Kumamoto Health Science University |
| Journal Type | Japan |
| Volume, Issue, Page | pp.77-86 |
| Total page number | 10 |
| Authorship | Lead author |
| Author and coauthor | Ippei NOBORUO, Keita KIMURA, Saki NAKAO
Ryoya MATSUKATA, Tatsuya KAWAGUCHI, Yukinori KOZUMA |
| Details | In the case of indirect antiglobulin tests, binding of daratumumab （DARA） to red blood cells, an
anti-CD38 antibody for the treatment of multiple myeloma （MM）, can return false-positive results. We attempt to develop a new detection method that avoids the interference of DARA without using dithiothreitol （DTT） in this study. To evaluate the combination of DARA and red blood cells （RBCs）, we examined CD38 expression in the presence of various concentrations of DARA using flow cytometry. Mean fluorescence intensity （MFI） of CD38 was decreased by the addition of DARA. Furthermore, the binding of anti-CD38 antibodies to RBCs inhibited in the presence of DARA was recovered by the removal of DARA. These results suggest that the interference of DARA can be avoided by the addition of His-tag conjugated recombinant human CD38 protein （H-rhCD38） to the serum of patients with MM during DARA treatment without using DTT. |