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Motohiro Takeya
Department Kumamoto Health Science University Department of Medical Technology, Faculty of Health Science Position Professor |
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| Research Period | 1996～1997 |
| Research Topic | Role of Monocyte Chemoattractant Protein-1 in Various Pathological Conditions |
| Research Type | KAKENHI Research |
| Consignor | Ministry of Education, Culture, Sports, Science and Technology |
| Research Program Type | Grants-in-Aid for Scientific Research(基盤研究(B)),基盤研究(B) |
| KAKENHI Grant No. | 08457073 |
| Representative Person | Motohiro TAKEYA |
| Collaborative Researcher | Kazuhisa MIYAKAWA, Kiyoshi TAKAHASHI |
| Details | Monocyte chemoattractant protein-1 (MCP-1) is one of the most potent chemoattractants for monocytes which belongs to the C-C chemokine group. In this project, we clarified the crucial role of MCP-1 in various pathological conditions in rats.1.Injection of recombinant rat MCP-1 into rat skin induced infiltration of TRPM-3-positive activated macrophages, whereas ED2-positive resident macrophages did not accumulate to the injection site.2.In rat models of pulmonary granulomatosis, we confirmed the expression of MCP-1 in the early phase of inflammation. Administration of nitric oxide synthase inhibitors significantly suppressed nitric oxide (NO) production and resulted in marked reduction monocyte/macrophage infiltration as well as in inhibition of MCP-1 production. These data indicate that NO may induce MCP-1 expression in lung granulomatosis.3.In collagen-induced rat arthritis, both MCP-1 concentration in the joint lavages and MCP-1 mRNA levels in the joint tissues at 2 weeks after the immunization of collagen. Injection of an anti-rat MCP-1 monoclonal antibody significantly decreased the number of exudate macrophages in the lesions and reduced the ankle swelling by about 30% compare |
| Permalink URL | https://kaken.nii.ac.jp/d/p/08457073.ja.html |