Motohiro Takeya
   Department   Kumamoto Health Science University  Department of Medical Technology, Faculty of Health Science
   Position   Professor
Research Period 1996~1998
Research Topic Pathological Significance of Macrophage Scavenger Receptors
Research Type KAKENHI Research
Consignor Ministry of Education, Culture, Sports, Science and Technology
Research Program Type Grants-in-Aid for Scientific Research(基盤研究(B)),基盤研究(B)
KAKENHI Grant No. 08457071
Representative Person Kiyoshi TAKAHASHI
Collaborative Researcher Tatsuhiko KODAMA, Kazuhisa MIYAKAWA, Motohiro TAKEYA
Details 1. Anti-human monoclonal antibodies for macrophage scavenger receptors (MSR), as well as polyclonal antibodies for synthetic peptides of MSR, were generated in our laboratory. Using these antibodies, our immunohistochemical and immunoelectron microscopic studies demonstrated that MSR was distributed on the cell membranes of macrophages ubiquitously distributed in various organs and tissues of humans. Immunohistochemical and immunoelectron microscopic investigation with anti-bovine or anti-murine MSR monoclonal antibodies revealed similar distribution and subcellar localization of MSR in bovine and murine tissues.2. Our studies demonstrated in humans, bovines, and mice that MSR can bind to modified low density lipoproteins (LDL) such as acetylated LDL (acLDL) or oxidized LDL (oxLDL) and to advanced glycation end products (AGE). MSR-deficient mice revealed marked reductions in the uptake of acLDL, oxLDL, and AGE.3. In atheroselerotic lesions of human aorta, macrophage-derived foam cells showed the expression of MSR and intracellular accumulation of oxLDL and AGE.In apo-E/MSR double knockout mice and diet-induced LDL receptor/MSR double knockout mice, the lesion size of atheroselerosi
Permalink URL