|
Motohiro Takeya
Department Kumamoto Health Science University Department of Medical Technology, Faculty of Health Science Position Professor |
|
| Research Period | 1998～1999 |
| Research Topic | Development of quantitative analysis method of the atherosclerotic lesions in gene targeted mice |
| Research Type | KAKENHI Research |
| Consignor | Ministry of Education, Culture, Sports, Science and Technology |
| Research Program Type | Grants-in-Aid for Scientific Research(基盤研究(B)),基盤研究(B) |
| KAKENHI Grant No. | 10557027 |
| Representative Person | Kiyoshi TAKAHASHI |
| Collaborative Researcher | 鈴木 宏志, Sakashita NAOMI, Motohiro TAKEYA, Hiroshi SUZUKI |
| Details | One of the most reliable methods for the quantitative analysis of atherosclerotic lesions is direct measurement of the lesion size on tissue sections. In this study, we selected the cross-sections through the aortic valve and ascending aorta where the atherosclerosis occurs at very early stage. Using the aortic valve as a marker, the place of the sectioning is easily oriented. In practice, the four slices crossing aortic valve and ascending aorta were selected, and the area of atherosclerosis in each section was measured by the image analysis microscope system.Using this method, it was quantitatively proven that the progress of the atherosclerosis in LDL receptor deficient mice was suppressed in the absence of macrophage scavenger receptor (MSR). Other scavenger receptors than MSR such as CD36, MARCO receptor, and CD68/Macrosialin were considered to concern in the lipid uptake of the macrophages at the remaining lesion. This method was also available for the evaluation of anti-atherosclerosis drugs. The measurement showed that a newly developed antioxidant (BO-653) was effective to reduce the atherosclerotic lesion size in ApoE deficient mice.Though the introduction of the present |
| Permalink URL | https://kaken.nii.ac.jp/d/p/10557027.ja.html |