Motohiro Takeya
   Department   Kumamoto Health Science University  Department of Medical Technology, Faculty of Health Science
   Position   Professor
Research Period 1998~2000
Research Topic Macrophage scavenger receptor as a new macrophage marker
Research Type KAKENHI Research
Consignor Ministry of Education, Culture, Sports, Science and Technology
Research Program Type Grants-in-Aid for Scientific Research(基盤研究(B)),基盤研究(B)
KAKENHI Grant No. 10470061
Representative Person Motohiro TAKEYA
Collaborative Researcher Naomi SAKASHITA, Kazuhisa MIYAKAWA, Kiyoshi TAKAHASHI
Details Macrophage scavenger receptor (MSR) is one of the major receptors of macrophages and play important roles in atherogenesis and host defense mechanisms. Monoclonal antibodies were generated by immunizing MSR-deficient mice with a recombinant protein of human typeI MSR as immunogen. Five antibodies were confirmed to be specific for MSR by Western blot analysis.Immunohistochemically, all antibodies recognized tissue macrophages such as alveolar macrophages, Kupffer cells in the liver, red pulp macrophages in the spleen, sinus macrophages in lymph nodes, and interstitial macrophages in various organs. Perivascular macrophages in the brain (Mato cells) were also positive for these antibodies. Freshly isolated blood monocytes were negative ; however, they became positive for these antibodies after 1 day in culture. At 3 to 5 days in culture the reaction intensity became stronger along their differentiation towards macrophages. Dendritic cells such as interdigitating cells of lymphoid tissues and epidermal Langerhans cells were invariably negative. In early atherosclerotic lesions, these antibodies recognized scattered macrophages in intima and foamy macrophages in the periphery of ather
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