ヒガシ　ヤスヒコ
  東　康彦
   所属   薬学部 薬学科
   職種   教授
言語種別 英語
発行・発表の年月 2008
形態種別 学術論文
標題 Simultaneous determination of N-dealkylated metabolites of four butyrophenone-type agents by HPLC with fluorescence detection after pre-column derivatization with dansyl chloride
執筆形態 共著
掲載誌名 J. Liq. Chromatogr. & Rel. Technol.
巻・号・頁 31(18),pp.2762-2770
概要 The basic metabolites of butyrophenone-type agents are sometimes more neurotoxic than the parent compounds. Recently, we developed a simple high-performance liquid chromatographic (HPLC) method coupled with dual ultraviolet detection to determine the N-dealkylated basic metabolites, i.e., 1,3-dihydro-1-(1,2,3,6-tetrahydro-4-pyridinyl)- 2H-benzimidazole-2-one, 1-phenyl-1,3,8-triazaspiro[4.5]decan-4-one, 4-(4-chloro- phenyl)-4-hydroxypiperidine, and 4-(4-bromophenyl)-4-hydroxypiperidine (BPHP), of droperidol, spiperone, haloperidol, and bromperidol, respectively, in phosphate-buffered saline (pH 7.4). An HPLC analysis with fluorescence detection was also developed to quantify these metabolites in rat plasma after pre-column derivatization with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F). In this study, dansyl chloride (Dns-Cl) was used for determination of these metabolites instead of NBD-F.