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オヤマダ マサヒト
Oyamada Masahito
小山田 正人 所属 藤女子大学 人間生活学部 食物栄養学科 職種 特任教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2005/07 |
| 形態種別 | 研究論文（学術雑誌） |
| 査読 | 査読あり |
| 標題 | Multiphoton excitation-evoked chromophore-assisted laser inactivation using green fluorescent protein |
| 執筆形態 | 共著 |
| 掲載誌名 | NATURE METHODS |
| 掲載区分 | 国外 |
| 出版社・発行元 | NATURE PUBLISHING GROUP |
| 巻・号・頁 | 2(7),pp.503-505 |
| 著者・共著者 | T Tanabe,M Oyamada,K Fujita,P Dai,H Tanaka,T Takamatsu |
| 概要 | Noninvasive, straightforward methods to inactivate selected proteins in living cells with high spatiotemporal resolution are needed. Chromophore-assisted laser inactivation (CALI) can be used to photochemically inactivate proteins, but it has several drawbacks, such as procedural complexity and nonspecific photodamage. Here we show that by application of multiphoton excitation to CALI, enhanced green fluorescent protein (EGFP) is an effective chromophore for inactivation of a protein's function without nonspecific photodamage in living mammalian cells. |
| DOI | 10.1038/NMETH770 |
| ISSN | 15487091 |
| PMID | 15973419 |
| PermalinkURL | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=23644433309&origin=inward |