ミツモト　ヤスヒデ   Yasuhide Mitsumoto
  光本　泰秀
   所属   薬学部 薬学科
   職種   教授
言語種別 英語
発行・発表の年月 2013/06
形態種別 学術論文
査読 査読あり
標題 Attenuation of nicotine-evoked Ca2+ influx by antibody to the nicotinic acetylcholine receptor α3 subunits in human embryonic kidney cells
執筆形態 共著
掲載誌名 Advances in Bioscience and Biotechnology
出版社・発行元 Scientific Research Publishing Inc.
巻・号・頁 4(6A),pp.9-14
著者・共著者 Shota Kobayashi, Shigeru Yokoyama, Takahiro Maruta, Akiko Muroyama, Hiroaki Yoshikawa, Yasuhide Mitsumoto
概要 Autoantibody against neuronal nicotinic acetylcholine receptor (nAChR) α3 subunit is implicated in severe autonomic dysfunction in the patients with autoimmune autonomic ganglionopathy (AAG). Although this autoantibody has been revealed to impair fast excitatory synaptic transmission in autonomic ganglia, its precise mechanism remains unknown. Here, we show that antibody-induced reduction of cell-surface α3 subunits result in impairment of nicotine-evoked Ca2+ influx in stably transfected human embryonic kidney cells. These effects of the antibody were remarkably inhibited by interfering with the endocytic machinery at low-temperature. We conclude that reduction of nAChR in autonomic ganglia can be mediated by the endocytosis of α3 subunits, and resulted in autonomic failure in AAG patients.
DOI 10.4236/abb.2013.46A002