ミツモト　ヤスヒデ   Yasuhide Mitsumoto
  光本　泰秀
   所属   薬学部 薬学科
   職種   教授
言語種別 英語
発行・発表の年月 1989/12
形態種別 学術論文
査読 査読あり
標題 Dual-fluorescence flow cytometric analysis of membrane potential and cytoplasmic free Ca2+ concentration in embryonic rat hippocampal cells
執筆形態 共著
掲載誌名 Cell Struct Funct
掲載区分国外
巻・号・頁 14,pp.669-672
担当区分 筆頭著者,責任著者
著者・共著者 Y Mitsumoto, T Mohri
概要 We have demonstrated simultaneous measurement of the membrane potential and cytoplasmic free Ca2+ concentration ([Ca2+]i) by utilizing a dual-laser flow cytometer in embryonic rat hippocampal cell suspensions. Veratrine, a Na+ channel activator, induced both membrane depolarization and elevation of [Ca2+]i. These actions of veratrine were all reversed by the presence of tetrodotoxin (TTX). These findings suggest that Na+ channels are functionally expressed in the cells and the activation of Na+ channels increases [Ca2+]i. The usefulness of the flow cytometric analysis in elucidating the expression of membrane functions in the embryonic central nervous systems (CNS) is discussed.