ミツモト　ヤスヒデ   Yasuhide Mitsumoto
  光本　泰秀
   所属   薬学部 薬学科
   職種   教授
言語種別 英語
発行・発表の年月 1991/12
形態種別 学術論文
査読 査読あり
標題 Characterization of the uptake of adenosine by cultured rat hippocampal cells and inhibition of the uptake by xanthine derivatives
執筆形態 共著
掲載誌名 Neurosci Lett
掲載区分国外
巻・号・頁 133,pp.275-278
著者・共著者 T Ohkubo, Y Mitsumoto, T Mohri
概要 Hippocampal cells were cultured in 24-well culture plates with enriched populations of neuron or glial cells. The [3H]adenosine uptake by 7-10-day cultures of these cells was dependent on temperature, but independent of extracellular Na+. The uptake of adenosine (10 microM) for 15 s was greatly blocked by addition of 100 microM dipyridamole, 50-200 microM propentofylline or 50 microM of 2-chloroadenosine or nitrobenzylthioinosine in both cells and by 100 microM pentoxifylline in neuron. Either caffeine or theophylline (50 microM each) had no effect on the uptake by these cells. Inhibition of the adenosine uptake by propentofylline was demonstrated to be competitive in both cells.