ミツモト　ヤスヒデ   Yasuhide Mitsumoto
  光本　泰秀
   所属   薬学部 薬学科
   職種   教授
言語種別 英語
発行・発表の年月 2003/08
形態種別 学術論文
査読 査読あり
標題 Honokiol and magnolol induce Ca2+ mobilization in rat cortical neurons and human neuroblastoma SH-SY5Y cells
執筆形態 共著
掲載誌名 Eur J Pharmacol
掲載区分国外
巻・号・頁 474,pp.199-204
著者・共著者 Haifeng Zhai, Kousuke Nakade, Yasuhide Mitsumoto, Yoshiyasu Fukuyama
概要 We examined the intracellular Ca(2+) response in primary cultured rat cortical neurons and human neuroblastoma SH-SY5Y cells by Fluo 3 fluorescence imaging analysis. In these two kinds of neuronal cells, honokiol and magnolol increased cytoplasmic free Ca(2+) with a characteristic lag phase. The cytoplasmic free Ca(2+) increase was independent of extracellular Ca(2+), but dependent on activation of phospholipase C and inositol 1,4,5-triphosphate (IP(3)) receptors. These results suggest that honokiol and magnolol increase cytoplasmic free Ca(2+) through a phospholipase C-mediated pathway, and that the release of Ca(2+) from intracellular stores mainly contributes to the increase in cytoplasmic free Ca(2+). Thus, honokiol and magnolol may be involved in a new activation mechanism closely associated with intracellular Ca(2+) mobilization.