ミツモト　ヤスヒデ   Yasuhide Mitsumoto
  光本　泰秀
   所属   薬学部 薬学科
   職種   教授
言語種別 英語
発行・発表の年月 2011/04
形態種別 学術論文
査読 査読あり
標題 Loss of striatal dopaminergic terminals during the early stage in response to MPTP injection in C57BL/6 mice
執筆形態 共著
掲載誌名 Neuroscience Research
出版社・発行元 Elsevier Ireland Ltd and the Japan Neuroscience Society
巻・号・頁 69(4),pp.352-355
著者・共著者 Akiko Muroyamaa, Shota Kobayashia and Yasuhide Mitsumoto
概要 The molecular mechanisms underlying MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine)-induced dopaminergic (DAergic) neuronal death in vivo are still not fully understood. To investigate the selective DAergic neurotoxicity, we have developed an immunological technique to isolate DAergic synaptosomes from mouse striatal tissues using an antibody against 20 amino acid residues in the extracellular second loop of dopamine transporter (DAT). The DAT protein level in the isolated DAergic synaptosomes was markedly decreased at 16 h after a single injection of 30 mg/kg MPTP, but not in striatal homogenate and crude synaptosomes fraction. GBR-12909, a dopamine uptake inhibitor, completely reversed the MPTP-induced decrease of DAT protein in the DAergic synaptosomes. These results suggest that the isolated DAergic synaptosomes can be useful to identify mechanisms of loss of the nerve terminals.
DOI 10.1016/j.neures.2010.12.009
ISSN 0168-0102